Application Case: Digital PCR in COVID-19 Diagnosis & Monitoring

ØHigh-sensitivity detection of SARS-CoV-2

Digital PCR is more accurate than RT-qPCR, decreasing the false-negative or ambiguous results, especially in patients with low viral loads.

ØDetection of SARS-CoV-2 mutations

Digital PCR is suitable for the detection of known SARS-CoV-2 mutations, especially for some low-frequency mutations.

ØDynamic monitoring of disease progression

Viral load in the early and progressive stages was significantly higher than that in

the recovery stage, suggesting that dynamic monitoring of the viral load helps to

assess the progression of the disease.

ØMonitoring the virus concentration in the environment

Using RT-qPCR and dPCR to detect virus remaining on the surface of

laboratory-related objects, all samples were found negative by RT-qPCR, while 13 of 61 samples were considered positive by dPCR.

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