Rat IL-1β(Interleukin 1 Beta) ELISA Kit

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-1β. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody.

Then a biotinylated detection antibody specific for Rat IL-1β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-1β, biotinylated antibody detection and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow.

The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-1β. You can calculate the concentration of Rat IL-1β in the samples by comparing the OD of the samples to the standard curve.
Product overview
Abcam’s IL-1 beta Rat ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-1 beta in cell lysates and tissue lysates.

This assay employs an antibody specific for rat IL-1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-1 beta antibody is added.

After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Enzyme-linked immunosorbent assay (ELISA) kits make it easier to use ELISAs for detecting molecules or performing ligand-binding assays successfully. Many ELISA kits use the detection enzymes horseradish peroxidase (HRP) or alkaline phosphatase (AP), along with chromogenic, fluorescent, or chemiluminescent substrates.

Alternative detection strategies, such as fluorescent tags or other detectable probes, are also accepted under the umbrella term of ELISAs if they are plate-based methods, regardless of whether they use reporter enzymes or antibodies for detection. Please contact us for product data sheet and other of our laboratory apparatus