Mouse Frataxin ELISA Kit (ab199078)

The Mouse Frataxin ELISA Kit (ab199078) is a 90 minute single wash sandwich ELISA designed for the quantitative measurement of Frataxin protein in cell culture extracts and tissue extracts. It uses our patented SimpleStep ELISA® technology. Quantify mouse frataxin with a sensitivity of 5 pg / ml.


SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This sandwich ELISA approach allows for the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the imaging section for more details. Our ELISA® SimpleStep technology offers several benefits:


- Single wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility of superior antibodies
- Fully validated in biological samples
- 96-well plate that can be broken into 12 x 8-well strips


A 384-well SimpleStep ELISA® microplate (ab203359) is available for use as an alternative to the 96-well microplate provided with the SimpleStep ELISA® kits.


TEST SPECIFICITY


This kit recognizes both native and recombinant mouse Frataxin protein in cell and tissue extract samples only.


Whole blood, saliva, urine, serum and plasma samples have not been tested with this kit.


REACTIVITY OF THE SPECIES


This kit recognizes the mouse Frataxin protein.


Human reactivity was determined to be 48%, by measuring 310 pg / ml of human recombinant protein and comparing the D.O. raw. values ​​to the crude mouse standard protein O.D. Values ​​analyzed at the same concentration and expressing the ratio as a percentage of the reactivity of the human protein to the reactivity of the mouse protein within the assay.

Notes
Mouse frataxin is a 23 kDa core encoded mitochondrial protein. The gene is located on chromosome 9 and is highly conserved during evolution. The gene is expressed in every cell, although at different levels in different tissues and during development. The specific role of frataxin is still unknown, but it has been shown to play a role in iron metabolism. Studies have shown that deletion of the frataxin gene in yeast results in the accumulation of iron in the mitochondria and loss of respiration. Recombinant frataxin has been shown to bind iron in vitro and increased mitochondrial iron levels have been observed in patients with Friedreich's Ataxia (FRDA). FRDA is an autosomal recessive progressive degenerative disease characterized by neurodegeneration and cardiomyopathy; it is the most common inherited ataxia.

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