Immunohistochemistry (IHC) is a histology technique aimed at locating a given protein in a tissue. There is also a derived technique, immunocytochemistry (ICC) which has the same principle but which aims to locate a given protein in isolated cells.
The principle of immunohistochemistry is based on the recognition of an antigen by a specific antibody and the revelation of this antigen-antibody complex by a chemical reaction thanks to an enzyme and its substrate. In general, the enzyme is HRP (Horse Radish Peroxidase) or AP (Phosphatase Alkaline) and their substrates AEC and DAB for HRP or NBT / BCIP and Permanent Red for alkaline phosphatase.
It is possible to locate a single protein (single labeling) or several proteins on the same tissue section (multiple labeling).
We offer a wide choice of products for immunohistochemistry (IHC):
Secondary antibodies biotinylated or directly coupled to the enzyme
Enzyme or enzyme complex
Buffers and related reagents