Human CLCF1 (Cardiotrophin-like cytokine factor 1) ELISA Kit - Information
The Assay Genie Human CLCF1 (Cardiotrophin-like cytokine factor 1) ELISA Kit can assay for Human CLCF1 in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.
How our Human CLCF1 (Cardiotrophin-like cytokine factor 1) ELISA Kits Work?
The Assay Genie (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today's scientists demand high quality consistent data for high impact journals, Assay Genie have developed our range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands. Our assay kits use a quantitative sandwich ELISA technique and each kit comes with highly specific antibodies pre-coated onto a 96-well microtiter plate.
At Assay Genie we understand the need for speed! Therefore, we have developed an ultra-fast protocol meaning you achieve your results rapidly. So, once you have prepared and plated your samples, blanks and standards, you simply incubate with a highly specific biotin-conjugated primary antibody and Avidin conjugated to Horseradish Peroxidase (HRP) and incubate for the appropriate length of time. After washing the plate according to the protocol and addition of the TMB (3,3 ', 5,5'-Tetramethylbenzidine) solution, the appearance of a blue color should be detected due to an enzymatic reaction catalysed by HRP. Next step is the addition of the Stop Solution which terminates the HRP reaction and the blue color turns yellow with the signal intensity measured on a plate reader at 450nm. The amount of bound Human CLCF1 is proportional to the signal generated by the reaction meaning the kit assay gives you a quantitative measurement of the analyte in your samples.
Human CLCF1 (Cardiotrophin-like cytokine factor 1) ELISA Kit Protocol
The below protocol is a sample protocol for Human CLCF1 (Cardiotrophin-like cytokine factor 1) ELISA Kit using a biotinylated detection antibody and streptavidin-HRP. Sandwich ELISA Kits allow for the detection and quantification of an analyte in a sample by using known analyte concentrations as standards and plotting absorbance of known concentrations vs known standard concentrations. This allows the researcher to calculate the amount of Human CLCF1 Antibody present in their sample.
Before adding to wells, equilibrate the SABC working solution and TMB substrate for at least 30 min at 37 ° C. When diluting samples and reagents, they must be mixed completely and evenly. It is recommended to plot a standard curve for each test.