Galic Acid Standard

€42.00
(No reviews yet) Write a Review
SKU:
BQC-ST08002
Availability:
Y
Adding to cart… The item has been added

Please note that BioQuochem items have a $300 minimum. Please contact us if you have any questions.



Gallic acid is a polyphenol produced by plants, which shows antioxidant properties. It is commonly used to measure polyphenol concentration but could also be used to measure antioxidant capacity. It is present in a wide variety of plant-based food samples.

RECOMMENDED USES

To compare the e-BQC results (µC) to classical antioxidant capacity units of Gallic acid Antioxidant Capacity Equivalents (GAE). It could be used for biological and food samples.

COMPATIBLE BUFFERS

ReferenceProductDescription
BQC-ST08006Bio-Buffer150 mM Sterile PBS pH 7.4
BQC-ST08007General Buffer100 Mm PBS pH 7
BQC-ST08005Nutrition Buffer0.1 M PBS pH 5.8

 

STANDARD CALIBRATION

Add 1 ml of the selected diluent buffer to the vial (10 mM). Transfer the 1 ml from the vial and add 9 ml of diluent (1 mM). Prepare the calibration curves in 1.5 ml tubes as shown below:

 

Standard (µl)

Diluent (µl)

GAE (µM)

1

0

1000

0

2

10

990

10

3

25

975

25

4

50

950

50

5

75

925

75

6

100

900

100

*This is just a recommendation in case the expected antioxidant capacity values of the sample were unknow. You are free to adapt the concentrations to be tested to your necessities.

e-BQC SPECIFICITY

Gallic acid is more predominantly present in Q2 where it has higher sensitivity. It is recommended to use Q2 for calibrations at low concentrations of Gallic acid.

 

Q1

Q2

Qt

Sensitivity

0.02 µC/ µM

0.05 µC/ µM

0.8 µC/ µM

LOD

5 µM

10 µM

7 µM

LOQ

17 µM

30 µM

25 µM

LOB

2 µM

12 µM

14 µM

RSD

15 %

10 %

14 %