Canine Haptoglobin ELISA Kit (ab137978)

Abcam’s Haptoglobin Canine in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit designed for the quantitative measurement of Haptoglobin levels in plasma, serum, and cell culture supernatants.

A Haptoglobin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Haptoglobin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer.

TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Haptoglobin captured in plate.